LOCUS       40924_36723        6802 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Inducible protein expression vector pReg Neo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Regulated Mammalian Expression System Technical Manual, TM289
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 6802)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6802)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Road, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        95..113
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      141..433
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
                     /regulatory_class="promoter"
     misc_feature    444..587
                     /label=6X lambda operator
                     /note="6X lambda operator"
     regulatory      600..659
                     /label=minimal CMV promoter
                     /note="minimal CMV promoter"
                     /regulatory_class="promoter"
     promoter        725..743
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1841..1861
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1889..2431
                     /label=p65
                     /note="C-terminal portion of the p65 subunit of mouse
                     NF-kappa-B"
     promoter        complement(2471..2488)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(2506..2627)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2813..3268
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3385..3742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3793..4584
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4651..4699
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        5005..5109
                     /label=AmpR promoter
     CDS             5110..5967
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6141..6729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggcttaact atgcgaccgg tgcctccata tggacatatt gtcgttagaa cgcggctaca
       61 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaactcgat
      121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      181 gcaaagcatg catctcaatt agtcagcaag gaaagtcccc aggctcccca gcaggcagaa
      241 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      301 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      361 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaagag
      421 gcttttttgg aggagatctc gagtttacct ctggcggtga tagtcgagtt tacctctggc
      481 ggtgatagtc gagtttacct ctggcggtga tagtcgagtt tacctctggc ggtgatagtc
      541 gagtttacct ctggcggtga tagtcgagtt tacctctggc ggtgatagtc gactctagat
      601 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      661 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagccta
      721 agcttaatac gactcactat agggagaccc aagctgggaa gcttccacca tgagcacaaa
      781 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga
      841 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg
      901 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc
      961 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga
     1021 aatctacgag atgtatgaag cggtagggat gcagccgtca cttagaagtg agtatgagta
     1081 ccctgttttt tctcatgttc aggcagggat gttctcacct aagcttagaa cctttaccaa
     1141 aggtgatgcg gagagatggg tagatatctc gaattcttat gactcctcca gtatcaaagt
     1201 cctgaaaggg ctggatgcgg tgcgtaagcg cccgggtatg tatatcggcg acacggatga
     1261 cggcaccggt ctgcaccaca tggtattcga ggtggtagat aacgctatcg acgaagcgct
     1321 cgcgggtcac tgtaaagaaa ttatcgtcac cattcacgcc gataactctg tctctgtaca
     1381 ggatgacggg cgcggcattc cgaccggtat tcacccggaa gagggcgtat cggcggcgga
     1441 agtgatcatg accgttctgc acgcaggcgg taaatttgac gataactcct ataaagtgtc
     1501 cggcggtctg cacggcgttg gtgtttcggt agtaaacgcc ctgtcgcaaa aactggagct
     1561 ggttatccag cgcgagggta aaattcaccg tcagatctac gaacacggtg taccgcaggc
     1621 cccgctggcg gttaccggcg agactgaaaa aaccggcacc atggtgcgtt tctggcccag
     1681 cctcgaaacc ttcaccaatg tgaccgagtt cgaatatgaa attctggcga aacgtctgcg
     1741 tgagttgtcg ttcctcaact ccggcgtttc cattcgtctg cgcgacaagc gcgacggcaa
     1801 agaagaccac ttccactatg aaggcggccc atgtatgggc cctaaaaaga agcgtaaagt
     1861 cgccatcgat cagctcacca tggtgtttcc ttctgggcag atctcaaacc aggccctggc
     1921 cttagcaccg tcctctgccc cagtccttgc ccagaccatg gtcccttcct cagccatggt
     1981 acctctggct cagcccccag ctcctgcccc agttctaacc ccgggtcctc cccagtccct
     2041 gtctgcacct gttccaaaga gcacccaggc tggggaaggc acgctgtcgg aagccctgct
     2101 gcacctgcag tttgatgctg atgaagactt gggggccttg cttggcaaca gcacagaccc
     2161 aggagtgttc acagacctgg catctgtgga caactcagag tttcagcagc tcctgaacca
     2221 gggtgtgtcc atgtctcact ccacagctga gcccatgctg atggagtacc ctgaagctat
     2281 aactcgcctg gtgacagggt cccagaggcc ccctgaccca gctcccacac ccctggggac
     2341 ctcggggctt cccaatggtc tctccggaga tgaagacttc tcctccattg cggacatgga
     2401 cttctctgct ctgctgagtc agatcagctc cagcggccaa taatctagag tcgacccggg
     2461 cggccgcttc cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg
     2521 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2581 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2641 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
     2701 acctctacaa atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga
     2761 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     2821 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2881 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2941 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3001 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3061 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3121 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3181 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3241 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     3301 gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga
     3361 aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca
     3421 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3481 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3541 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3601 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3661 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3721 tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaacttaag
     3781 gctagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3841 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3901 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3961 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4021 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4081 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4141 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4201 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4261 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4321 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4381 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4441 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4501 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4561 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4621 cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg
     4681 tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg cactctcagt
     4741 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     4801 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4861 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4921 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5641 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6781 gctcacatgg ctcgacagat ct
//
